LOCUS       40924_11476       10439 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gene trap vector pCMT-SAhygpA-NP22 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Horie K, Kokubu C, Yoshida J, Akagi K, Isotani A, Oshitani A, Yusa 
            K, Ikeda R, Huang Y, Bradley A, Takeda J.
  TITLE     A homozygous mutant embryonic stem cell bank applicable for 
            phenotype-driven genetic screening
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10439)
  AUTHORS   Horie K, Takeda J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2011) Contact:Kyoji Horie Osaka University, 
            Department of Social and Environmental Medicine, Graduate School of 
            Medicine; 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
REFERENCE   3  (bases 1 to 10439)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10439)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JAN-2011) Contact:Kyoji Horie Osaka University, Department of 
            Social and Environmental Medicine, Graduate School of Medicine; 2-2 
            Yamadaoka, Suita, Osaka 565-0871, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        745..1124
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1125..1328
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    1342..1409
                     /label=R region of Moloney Murine Leukemia Virus
                     /note="R region of Moloney Murine Leukemia Virus"
     misc_feature    1410..1484
                     /label=U5 region of Moloney Murine Leukemia Virus
                     /note="U5 region of Moloney Murine Leukemia Virus"
     misc_feature    1549..1906
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1965..2381
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    2391..2765
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     polyA_signal    complement(2801..3025)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3036..3069)
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             3086..3679
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             3686..4678
                     /codon_start=1
                     /label=Delta-TK
                     /note="truncated herpes simplex virus thymidine kinase
                     (Salomon et al., 1995)"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             complement(4685..5485)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    5502..5535
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        complement(5540..6038)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     misc_feature    complement(6065..6275)
                     /label=contains phosphoglycerate kinase 1 polyA signal
                     /note="contains phosphoglycerate kinase 1 polyA signal"
     CDS             complement(6282..7304)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    complement(7339..7442)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     protein_bind    complement(7819..7852)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    8052..8119
                     /label=R region of Moloney Murine Leukemia Virus
                     /note="R region of Moloney Murine Leukemia Virus"
     misc_feature    8120..8194
                     /label=U5 region of Moloney Murine Leukemia Virus
                     /note="U5 region of Moloney Murine Leukemia Virus"
     promoter        complement(8251..8269)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8290..8306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8314..8330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8338..8368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8383..8404)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8692..9280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9454..10311)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10312..10416)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggcctg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
      721 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
      781 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      841 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      901 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      961 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1021 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1081 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1141 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1201 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1261 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1321 gcagagctcg tttagtgaac cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     1381 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     1441 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
     1501 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggccagc
     1561 aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg
     1621 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg
     1681 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc
     1741 gacctgagtc caaaaatccc gatcgttttg gactctttgg tgcacccccc taataggagg
     1801 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
     1861 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt
     1921 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca
     1981 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
     2041 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
     2101 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
     2161 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
     2221 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
     2281 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
     2341 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga
     2401 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
     2461 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
     2521 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
     2581 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
     2641 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
     2701 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
     2761 gactgccgga tctagctagt tatcgagtcc gcctcagaag ccatagagcc caccgcatcc
     2821 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
     2881 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
     2941 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
     3001 gatggctggc aactagaagg cacagtcgag gctagataac ttcgtatagg atactttata
     3061 cgaagttatt ggatccacca tggggaccga gtacaagccc acggtgcgcc tcgccacccg
     3121 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     3181 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     3241 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     3301 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     3361 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     3421 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     3481 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     3541 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     3601 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     3661 gacccgcaag cccggtgccg gctccatgcc cacgctactg cgggtttata tagacggtcc
     3721 ccacgggatg gggaaaacca ccaccacgca actgctggtg gccctgggtt cgcgcgacga
     3781 tatcgtctac gtacccgagc cgatgactta ctggcgggtg ctgggggctt ccgagacaat
     3841 cgcgaacatc tacaccacac aacaccgcct cgaccagggt gagatatcgg ccggggacgc
     3901 ggcggtggta atgacaagcg cccagataac aatgggcatg ccttatgccg tgaccgacgc
     3961 cgttctggct cctcatatcg ggggggaggc tgggagctca catgccccgc ccccggccct
     4021 caccctcatc ttcgaccgcc atcccatcgc cgccctcctg tgctacccgg ccgcgcggta
     4081 ccttatgggc agcatgaccc cccaggccgt gctggcgttc gtggccctca tcccgccgac
     4141 cttgcccggc accaacatcg tgcttggggc ccttccggag gacagacaca tcgaccgcct
     4201 ggccaaacgc cagcgccccg gcgagcggct ggacctggct atgctggctg cgattcgccg
     4261 cgtttacggg ctacttgcca atacggtgcg gtatctgcag tgcggcgggt cgtggcggga
     4321 ggactgggga cagctttcgg ggacggccgt gccgccccag ggtgccgagc cccagagcaa
     4381 cgcgggccca cgaccccata tcggggacac gttatttacc ctgtttcggg cccccgagtt
     4441 gctggccccc aacggcgacc tgtataacgt gtttgcctgg gccttggacg tcttggccaa
     4501 acgcctccgt tccatgcacg tctttatcct ggattacgac caatcgcccg ccggctgccg
     4561 ggacgccctg ctgcaactta cctccgggat ggtccagacc cacgtcacca cccccggctc
     4621 cataccgacg atatgcgacc tggcgcgcac gtttgcccgg gagatggggg aggctaactg
     4681 atcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     4741 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     4801 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     4861 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     4921 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
     4981 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     5041 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     5101 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     5161 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     5221 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     5281 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     5341 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     5401 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     5461 catcttgttc aatggccgat cccatggtgg cggcgtcgac aataacttcg tataaagtat
     5521 cctatacgaa gttataggtc gaaaggcccg gagatgagga agaggagaac agcgcggcag
     5581 acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc
     5641 cgcccctgag cccgcccctg agcccgcccc cggacccacc ccttcccagc ctctgagccc
     5701 agaaagcgaa ggagcaaagc tgctattggc cgctgcccca aaggcctacc cgcttccatt
     5761 gctcagcggt gctgtccatc tgcacgagac tagtgagacg tgctacttcc atttgtcacg
     5821 tcctgcacga cgcgagctgc ggggcggggg ggaacttcct gactagggga ggagtagaag
     5881 gtggcgcgaa ggggccacca aagaacggag ccggttggcg ctaccggtgg atgtggaatg
     5941 tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca
     6001 tgctccagac tgccttggga aaagcgcctc ccctacccgg tagaattgcg gccagcttgc
     6061 ggccatccaa ctatgaaaca ttatcataaa gcaatagtaa agagccttca gtaaagagca
     6121 ggcatttatc taatcccacc ccacccccac ccccgtagct ccaatccttc cattcaaaat
     6181 gtaggtactc tgttctcacc cttcttaaca aagtatgaca ggaaaaactt ccattttagt
     6241 ggacatcttt attgtttaat agatcatcaa tttctcgact attcctttgc cctcggacga
     6301 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
     6361 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
     6421 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
     6481 tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc
     6541 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
     6601 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
     6661 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
     6721 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
     6781 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
     6841 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
     6901 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg
     6961 accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
     7021 gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc
     7081 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
     7141 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
     7201 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
     7261 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatggtggc ggcggctagc
     7321 gataccgtcg aattccccac tggaaagacc gcgaagagtt tgtcctcaac cgcgagctgt
     7381 ggaaaaaaaa gggacaggat aagtatgaca tcatcaagga aaccctggac tactgcgccc
     7441 tacagatctg cagcccgggg gatccactag ttctagcctc gagtagtcca atttgttaaa
     7501 gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct
     7561 atagagtacg agccatagat aaaataaaag attttattta gtctccagaa aaagggggga
     7621 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     7681 tggaaaaata cataactgag aatagagaag ttcagggaag cggggcgcat catatcaaat
     7741 gacgcgccgc tgtaaagtgt tacgggacat ctactttgtg catgacacaa gccagtgggt
     7801 cagaagttta catacactga agttcctata ctttctagag aataggaact tcttgagaaa
     7861 gaattgggat cccgatcaag gtcaggaaca gatggaacag ctagagaacc atcagatgtt
     7921 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     7981 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     8041 cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     8101 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     8161 gagtgattga ctacccgtca gcgggggtct ttcacccaga gtttggaact tactgtcttc
     8221 ttgggagggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     8281 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8341 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8401 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8461 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8521 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8581 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8641 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8701 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8761 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8821 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8881 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8941 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9001 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9061 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9121 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9181 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9241 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9301 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9361 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9421 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9481 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9541 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9601 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9661 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9721 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9781 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9841 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9901 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9961 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10021 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10081 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10141 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10201 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10261 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10381 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
